Vol. 2, Issue 7 (2016)
Reactive Oxygen Species (ROS) could be a causative factor for perfume-induced testicular toxicity in male rats
Author(s): Akunna GG, Akingbade AM, Faeji CO, Oni OI Akande OO
Abstract: Fragrance materials have been implicated in male infertility. These ingredients are unsteady and tend to oxidize easily. The dearth of information regarding the mechanism of action in which it induces testicular toxicity prompted us to evaluate its possible mechanism pathway in animal model. Sixty adult male wistar rats were randomly divided into six groups of ten rats each. Group A and B rats (Controls) rats were exposed (6hrs day-1) to 5 ml kg-1 body weight of normal saline for 56 days and 112 days via whole body inhalation respectively, Group C and D rats were exposed (6hrs day-1) to 5 ml kg-1 body weight of one of the perfume designated as F1 for a period of 56 days and 112 days via whole body inhalation respectively while Group E and Group F rats were exposed (6hrs day-1) to 5 ml kg-1 body weight of one of the perfume designated as F2 for a period of 56 days and 112 days via whole body inhalation respectively. Result obtained showed a significant decrease in the activity level of SOD, CAT, GPx and GSH and a significant elevation in the level of MDA when compared to values from the control groups of rat. The decrease in level of these testicular enzymatic and testicular non-enzymatic antioxidants (SOD, CAT, GPx and GSH and MDA respectively) in group exposed for 112 days (D and F) were more significant (P<0.005) when compared to groups of rats exposed for 56 days (C and E). However, the GSH content in group E rats was not significantly (P>0.05) different from that of the control groups of rats. From our study, we can only conclude that oxidative stress in testicular tissue might be responsible, at least in part, for perfume induced toxicity in animal model.