Vol. 3, Issue 3 (2017)
Nucleolar organizer regions (AgNORs) as a proliferative index in benign, premalignant and malignant colorectal lesions
Author(s): Dr. Nabeia Ali Gheryani, Dr. Houssein Elmatri
Abstract: Aims and Objectives: To establish the role of AgNORs in differentiating the benign from the pre-malignant and the malignant lesions of the colorectal. To establish the degree / grade of malignancy according to the AgNOR count. Material and methods: A retrospective study was conducted on 35 cases from July 2004 – July 2006 on the biopsies which were obtained from colorectal lesions. Two slides were prepared for each case – one was stained with the haematoxylin and eosin stain and the other was subjected to silver staining. The data was analyzed by using the independent t-test and ANOVA for the intergroup comparisons. Result: The results showed that the mean AgNOR count of the carcinomas was significantly higher than that of the benign tumours and inflammatory lesions (p < 0.05). The AgNOR dots tend to be small, homogenously stained and regular in the benign lesions but the dots became irregular, large dots or bizarre clusters in carcinoma and adenoma with dysplasia. Conclusions: AgNOR staining can be considered as a useful addition to diagnostic pathology. This study was helpful in the detection of the importance of AgNORs in differentiating the benign, pre-malignant and malignant lesions of the colorectum and it could be considered as an important tool along with the histopathological criteria for the evaluation of the proliferative activity of the cell.