At the time of menopause female sexual cycle ceases and female sex hormones diminish rapidly. It is characterized by various physiological, psychological and biochemical changes, and also metabolic bone disorders. Therefore in the present study we analyzed the level of aspartate aminotransferase, alanine aminotransferase, serum proteins, along with calcium and phosphorus levels in premenopausal women (n=40) and postmenopausal women (n=40). In premenopausal women the concentration of aspartate aminotransferase was elevated significantly as compared to postmenopausal women (p<0.001) but, the concentration of alanine aminotransferase was increased non significantly. The concentration of total serum protein increases in post menopausal women non significantly as compared to pre menopausal women’s whereas, the albumin level was decreased significantly in post menopausal women (p<0.0001). The serum calcium level was declined significantly in post menopausal women as compared to pre menopausal women’s (p<0.0001). Thus our study demonstrated that, as age advances liver gets irritated and the liver functions gets disturbed. The decreased concentration of calcium in postmenopausal women indicates that they are more prone to fractures and osteoporosis.