Study of umbilical cord blood culture in diagnosis of early-onset sepsis among newborns with high-risk factors
CD Aundhakar, Harshada Tatiya, Geeta Karande, Akhila S, Madhura K
Introduction: Neonatal sepsis is most common cause of neonatal mortality. Umbilical cord is a less commonly used site for collection of blood culture. Objectives: To analyze correlation of UCBC (Umbilical Cord Blood Culture) with Sepsis Screen and PVBC (Peripheral Vein Blood Culture) in Early Onset Neonatal Sepsis and also to compare organisms identified by UCBC and PVBC in neonatal sepsis. Methodology: A cohort of 75 neonates with perinatal infection risk factors was followed up prospectively. In the delivery room a blood sample from the umbilical vein was collected for culture. Peripheral venous blood was also collected for culture. All enrolled neonates were followed till their hospital stay. Analysis: Sepsis screen and UCBC were compared with PVBC to find out their sensitivity and specificity. Results: Among the 75 high risk neonates, sepsis screen was found positive in 24 (32%) while UCBC and PVBC were found positive in 13 (17.3%) and 4 (5.3%) respectively. Pseudomonas was the most commonly found organism in culture. Sepsis screen and UCBC had sensitivity of 100% and 75% respectively while specificity was 71.83% and 85.92% respectively when compared to PVBC. Conclusion: UCBC has good diagnostic validity for etiological diagnosis of bacterial sepsis in high risk neonates. Organisms grown in umbilical cord blood samples are comparable with venous blood culture samples. UCBC could be a painless, kinder, gentle approach instead of painful collection of blood by pricking the neonate.