Vol. 4, Issue 8 (2018)
Use of Gliricidia sepium aqueous leaf extract as an antisickling agent: Oxidative stress biomarkers in wistar rats exposed to the extract
Author(s): Taofeeq Oduola, Rabiu Aliyu Umar, Balarabe Adamu Isah, Mustapha Bello, Fatimah Aiyelabegan, Luqman Olatubosun Isa, Ganiyat Busayo Oduola
Abstract: Gliricidia sepium aqueous leaf extract is being used in the management of sickle cell disease without regard for harmful effects; hence this study was aimed at assessing the effect of ingestion of Gliricidia sepium leaf extract on antioxidant parameters and lipid peroxidation in Wistar rats. The acute oral toxicity of the leaf extract (LD50) and sub-chronic toxicity studies were determined according to Organization for Economic Cooperation and Development (OECD) Limit test 423 and OECD 407 guidelines respectively. Markers of lipid peroxidation and antioxidant factors were assayed using standard techniques at the end of the procedures. In the acute toxicity test, no death or sign of toxicity was observed for up to 14 days, vitamin C level and SOD activities were significantly higher (p<0.05) in experimental rats than the control but there was no significant difference between the test group and control for other parameters. In the sub-chronic toxicity study, there was no significant difference (p>0.05) between the control and the experimental rats for all the parameters except for SOD which was significantly higher (p<0.05) in the experimental rats than the control. In conclusion, Gliricidia sepium aqueous leaf extract is relatively safe for human consumption and may not induce oxidative stress imbalance.