Vol. 5, Issue 1 (2019)
Assessment of nucleic acid amplification test in blood donors in Darbhanga medical college and hospital
Author(s): Dr. Ashish Kumar Jha, Dr. Harishankar Mishra, Dr. Ajit Chaudhary
Abstract: A nucleic acid test (NAT) or nucleic acid amplification test (NAAT) is a technique utilized to detect a particular nucleic acid, virus, or bacteria which acts as a pathogen in blood, tissue, urine, etc. The NAT system differs from other tests in that it detects genetic materials rather than antigens or antibodies. Detection of genetic materials allows an early diagnosis of a disease because the detection of antigens requires time for antigens to appear in the bloodstream. Hence based on above findings the present study was planned to compare the sensitivity of NAT by using individual test and serological assays by using enzyme immunoassay. The study was planned in the Department of pathology, Darbhanga Medical College and Hospital, Darbhanga, Bihar from Jan 2018 to Oct 2018. Total 4500 patients have donated the blood and Out of that 60 patients were enrolled in the present study. The blood samples were evaluated for the Nucleic Acid Test (NAT). Approval of the institutional ethical committee was taken prior to conduct of the study. ELISA and NAT reports of HIV, HBV and HCV were collected in a specially designed proforma from the blood bank records. Both the tests are routinely done on the donor blood samples. The data generated from the study presented important data about nuclear acid test (NAT) testing of blood donors. Effectiveness of nucleic acid testing for blood donors screening is a debating area in transfusion medicine. Wide-national study is required to assess the safety and cost-effectiveness of using traditional and NAT testing to screen blood donors.
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