Introduction: Burn wounds are the site to bacterial colonization or infection, leading to morbidity and mortality. Early identification of microbial flora provides improved management and better prognosis. Purpose: The aim of this study was to identify the dynamics of bacteriological profile of burn cases admitted in the burn unit of SMS Medical College Jaipur. A prospective study was done in the bacteriology laboratory of the department of microbiology for 9 months. Methods: Swabs were collected from the wounds of a total of 396 pus swabs on admission, on 3rd day of admission and on 7th day from 122 burn cases admitted in burn unit, after taking a thorough history and demographic data. The swabs were inoculated in appropriate culture media, and identification was done following the standard procedure. Results: Among positive cultures(93.18), Pseudomonas sp. is the most common gram negative organism from 1st to 7th day, dominating in both GPC & GNB groups and Staphylococcus aureus was the common gram positive organism isolated during 1stday (1.32%), All gram positive organism were negligible by end of the 7th day. Pseudomonas with 95.19 % resistant to Ceftazidime may be potential ESBL producer. CPS are 100% resistant to Cefoxitin are MRSA. Conclusion: The finding of the study will be helpful for identifying the dynamics of common bacteria causing burn wound infection and also to take proper precautions to prevent the emergence of antibiotic-resistant bacteria by formulating an effective antibiotic policy with the usage of combinational drugs in the management of burn wound infections.