Vol. 5, Issue 5 (2019)
Carbapenem resistance among Escherichia coli in a tertiary care Rural Hospital
Author(s): Dardi Charan Kaur, Dr Jaishree Petkar
Abstract: The worldwide emergence of resistance to the powerful antibiotic carbapenem constitutes an important growing public health threat. Bacteria having carbapenemase have the potential to spread rapidly within the hospital environment and also across continents. so, the present study was carried out with the Aim to find the prevalence of Carbapenem resistance & Metallo-β-lactamases (MBLs) in Escherichia coli in rural hospital. And the antimicrobial resistant pattern of Carbapenem resistance & Carbapenem sensitive Escherichia coli. Methods: The present study was carried out in the Department of Microbiology from the period of January 2012 to August 2013. 294 Escherichia coli isolated from various clinical specimens were tested for carbapenem resistance by using meropenem disc [10µg] and by Modified Hodge test. The test was performed on all isolates on Mueller Hinton agar in accordance with guidelines of the CDC & CLSI. Metallo-β-lactamase detection was done. Isolates were tested for antibiotics by Kirby-Bauer disc diffusion method on Mueller-Hinton agar as per CLSI guidelines. Result: Of 294 Escherichia coli isolated from various clinical specimen, Carbapenem resistance was observed in 31(10.54%) & none of the isolates were positive for Metallo-β-lactamase by phenotypic method. Among 31 Carbapenem resistant isolates, Maximum Carbapenem resistance were from urine (35.48%) followed by stool (19.35%), Pus (19.35%), Misc (16.12%), Sputum (6.45%) &Blood (3.22%). Ward-wise maximum Carbapenem resistance were from medicine (32.25%) followed by ICU (22.58%), Paediatric (19.35%), Surgery (12.9%), Obgy (9.67%) & OPD (3.22%). High antibiotic resistance was seen in carbapenem resistant E. coli. Conclusion: Laboratories should routinely check for carbapenemase production among clinical isolates by phenotypic or genotypic methods.