Vol. 5, Issue 5 (2019)
in vitro antifungal Susceptibility testing of Dermatophytes isolated from clinical samples in tertiary care hospital
Author(s): Hemendra Kumar Sharma, Nitya Vyas, Ramesh Kumar Mishra, Babita Sharma
Abstract: Background: Dermatophytosis is colonization by dermatophytic fungus of the keratinized tissues like hair, nails and skin. They are considered important as a public health problem as well. The term ‘tinea’ is synonyms of word dermatophytosis. Depending upon the anatomical site of lesion, dermatophytes are classified into different varieties. Objective: To isolate, identify, and detect the in-vitro antifungal sensitivity pattern of various dermatophytes isolates, from clinically suspected cases of dermatophytosis Material and Methods: To find out antifungal resistance pattern among dermatophytes, 240 patients of all age group with clinical diagnosis of dermatophytosis were included under study. Identification and isolation was done by various test like macroscopic, microscopic, biochemical test and culture. Antifungal susceptibility testing was done by Agar Based Disc Diffusion (ABDD) Result: Out of 240 samples 100 isolates of dermatophytes were grown on fungal culture. Tinea ungium followed by tinea cruris and tinea corporis were common types. Trichophyton mentagrophytes (52%) and T. rubrum (28%) were most common species. Out of six antifungal used in our study fluconazole and clotrimazole were found most resistant while voriconazole was most effective drug. Conclusion: The epidemiology of dermatophyte infections may change with time. The Agar-Based Disk Diffusion method of antifungal susceptibility testing will aid the clinician in initiating prompt and appropriate antifungal therapy and prevent emergence of resistance.
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