Evaluation of diagnostic efficacy of GeneXpert MTB/RIF assay with Ziehl Neelsen staining & microscopy in pulmonary tuberculosis
Dr. Priyadarshini, Dr. Dhirendra Kumar
Tuberculosis is a bacterial highly chronic prevalent infectious disease caused by an intracellular aerobic bacterium known as mycobacterium tuberculosis. Because of this characteristic it prefers tissues which are always in contact with high oxygen levels as in lungs. Conventional methods for TB diagnosis such as microscopy have low sensitivity. Though culture is considered as gold standard, it is time consuming. Newer molecular diagnostic methods such as GeneXpert assay are rapid and highly sensitive and are now playing a pivotal role in TB diagnosis. The rapid detection of Mycobacteria in clinical specimens is essential for the early diagnosis and treatment of patients. Hence based on above data the present study was planned for Evaluation of diagnostic efficacy of GeneXpert MTB/RIF assay with Ziehl Neelsen Staining & Microscopy in Diagnosis of Pulmonary Tuberculosis. The present study was planned in Department of Microbiology, Aiims, Patna, Bihar, India. The study was conducted from September 2016 to august 2017. In the present study 660 suspected cases of the Pulmonary tuberculosis were included and evaluated. The data generated from the present study concludes that GeneXpert MTB/RIF assay is an effective tool for diagnosis of tuberculosis and has better sensitivity than smear and microscopic examination. It is very much useful to detect more cases of smear negative TB. It can also detect rifampicin resistance in TB bacilli (Multi-drug resistant tuberculosis).