Background: The Covid-19 pandemic has disrupted routine work worldwide and health care is obviously one of the most worst affected area. Frontline healthcare worker are at high risk of infection as they work in close proximity to the patients who may be infected with the Corona virus contributing to further spread. Aims: This study was planned to assess the incidence and clinical profile of COVID-19 infection among health care workers in a non COVID tertiary cancer hospital and also to analyze the effect of gender on the clinical spectrum of the disease. Methods and Materials: This is a retrospective analysis of all healthcare workers of Mahavir cancer sansthan and research centre, Patna who tested positive for Covid-19 by RT-PCR method 1st June 2020 to 31st July 2020. A 10 point questionnaire was prepared for data collection over telephonic and direct interview. Data was analyzed using Chi-square test and paired-t test. Results: 103 (14%) of the health care workers were infected with COVID-19 till 31st July, 2020 with a male to female ratio of 1:1.7. 46% and 38% of the HCW infected were between 31-40 years followed by cases in the 21-30 years age group respectively. 60% of the patients belonged to the nursing and housekeeping category. 41% of the total patients were asymptomatic at the time of diagnosis and 25% of the diagnosed cases remained asymptomatic throughout. The most common symptom was fever (29%) followed by myalgia (20%), sore throat (18%) and others. More number of female patients remained asymptomatic than the male counterpart but male patients showed a significantly faster recovery than the female patients. Conclusion: A high proportion of asymptomatic infection in health care workers stresses the proper use of PPE kits, prophylactic drugs along with routine screening for Covid-19 for protection of patients and proper functioning of the hospital.