Aim: To evaluate fungal and bacterial isolates of corneal ulcers. Material and method: A hospital based prospective study was carried out on 100 patients presenting with corneal ulcers at Department of Ophthalmology at Dr S.N.Medical College, Jodhpur from January 2019 to May 2019. Clinically suspected cases of fungal and bacterial corneal ulcers were selected for study and after diagnosis of corneal ulcers, a standardized Performa was filled. In all patients scrapings from corneal ulcers were subjected to complete microbiological evaluation. This was diagnosed microbiologically in two ways i.e. Smear examination by light microscopy and Culture. Results: 61% of patients are affected from bacterial infections, among them 95% patient has single strain & 5% has double strain. In 23% patient fungi is isolated. Fungal culture was positive in 83.3% patients. Fusarium sp (50%) is the most common fungi isolated, followed by Aspergillus sp (25%). Both are accounting nearly 75% of cases. Bacterial culture is positive in 88% of cases. The common bacterial isolates are Staphylococci Aureus (42%) and Staph Albus (285), followed by Pseudomonas (13.6%). Conclusion: The results of the study concluded that bacterial and fungal culture positive was 88% and 83.3% respectively.