Central line-associated bloodstream infection (CLABSI) is one of the most frequent and lethal complications of central venous catheterization. The need for this study lies in the fact that CLABSI in ICUs is common; it increases the length of hospital stay while increasing the mean attributable cost of treatment. Materials and Methods: A prospective observational study was conducted in the Central ICU (CICU) of Assam Medical College and Hospital (AMCH) after getting institutional ethical committee (IEC) approval. 100 patients were included in the study.Statistical Analysis: Statistical analysis was done using Microsoft Excel and Microsoft Word. Descriptive data were presented as mean ± SD. Categorical data were given as a percentage. Chi-square test was used for univariate analysis. Multiple logistic regression was used to determine predisposing risk factors. For all tests, p-value ˂ 0.05 was considered significant.Results: Nine patients developed CLABSI. CLABSI was seen after 8 days of catheterization. CLABSI rate was 7.8 per 1000 central line days. Klebsiella Pneumoniae (gram-negative) was the most common isolate.Conclusion: The high CLABSI rate in our ICU calls for the need for antibiotic stewardship program and tighter bundle care protocol.