The discovery of Helicobacter pylori (H. pylori) by Warren and Marshall in 1982 supported the increasing prevalence of H. pylori infection from year to year. H. pylori infection is associated with gastritis, peptic ulcer, gastric carcinoma, and MALT lymphoma. Often found in the gastric antrum, H. pylori colonise by producing urease to change the acid pH in the stomach (1.0-2.0) to the optimal pH to grow (6.7-7.0). The study found that patients who had the most gastric biopsy at MRCCC Siloam Hospitals Semanggi in 2014 were 92 patients. Of all data, most were found in female patients (59.3%) who had gastric endoscopic biopsies. Patients positively infected with H. pylori were 32 patients (8.6%), of whom 17 were WARD patients, and 15 outpatients were outpatients. Of the 32 positive patients, the majority suffered from non-atrophic active chronic gastritis, as many as ten patients. The results of this study are expected to be good information and knowledge for MRCCC Siloam Hospitals Semanggi and society.