Background: Osteoarthritis (OA) is a chronic degenerative joint disease that involves progressive softening and loss of articular cartilage, subchondral bone sclerosis, cyst formation and the development of osteophyte. OA knee causes the cartilage of the knee joint to thin and the joint surface to become rougher, causing the knee to move less smoothly and feel painful and stiff. Among multiple predisposing factors, occupation is one of the important factors that has been linked to OA. So, the knee joint load due to different occupations was studied. Objective: The study's objective was to evaluate the prevalence of knee pain caused by osteoarthritis in relation to various occupations. Methods: The study was conducted at the Bangladesh Korea Friendship Hospital's Pain Clinic in Dhaka, Bangladesh, to determine the occupational predisposing factors of knee osteoarthritis. A total of 201 cases were chosen by random sampling, with knee pain due to osteoarthritis changes of different occupations from July 2019 to June 2020. The results were statistically analyzed with Statistical Packages for Social Sciences (SPSS-24). Results: The age distribution of the patients shows that the majority of the patients 48(23.88 %) are between the ages of 45 and 49 years. The overall prevalence of knee pain in women is higher than in men. Women had high rates of prevalence for 49(24.38%) garments worker (tailoring) while high rates of prevalence in men were apparent for 32(15.92%) construction workers. Prevalence of knee pain in women 49(24.38%) were Garments worker (Tailoring), 20(9.96%) were Nurse, 15(7.46%) were Cleaner, 9(4.48%) were teachers, 5(2.48% were Construction workers, 3(1.49%) were Sales Assistant and 1(0.49%) were the driver. Prevalence of knee pain in men 26(12.93%) were Garment’s worker, 3(1.49%) were teacher, 35(15.92%) were Construction worker, 7(3.48%) were Sales Assistant, 15(7.46%) were driver, 10(4.98%) were carpenters and 6(2.99%) were Police/Security. The recent prevalence of knee symptoms was 157(78.11%) had pain, 18(8.96%) had stiffness, 16(7.95%) had swelling, 4(1.99%) had locked, and 6(2.99%) had any symptom. Conclusion: The result suggested that occupation might affect the development of chronic knee pain. In garments workers (tailoring), construction workers and carpenters are at increased risk of development of the knee OA because they are more vulnerable to develop joint trauma by bending of the knee and heavy lifting. So proper measures should be taken to reduce joint load.