International Journal of Medical and Health Research

Authors
Mirzadi Sadaf, Alka Rao, Afsha, Sheetal Sharma

Abstract
Pseudomonas aeruginosa has become an importantrnnosocomial and opportunistic pathogen in the second half of last century. Thernaim of the study was to isolate and asses the antimicrobial susceptibility ofrnPseudomonas aeruginosa at NIMS Medical College & Hospital, Jaipur. Totalrn300 samples were tested and 40 Pseudomonas aeruginosa were isolated. ThernPseudomonas aeruginosa were isolated using a standard protocol which includedrntests for motility, oxidase production, catalase production, biochemical reactions,rnoxidation- fermentation test, amino acids activity, nitrate reduction, malonaterntest and gelatin hydrolysis. Antimicrobial susceptibility were performed byrnKirby-Baeur disc diffusion method and result interpreted according to CLSIrnguidelines. The resistance pattern of Pseudomonas aeruginosa among variousrnantimicrobials are Meropenem (43%), Imepenem (0%), Piperacillin+tazobactamrn(20%), Polymyxin-b (53%), colistin (30%), Tigecycline (83%), amikacin (53%),rnAmpicillin+salbactum (85%), ciprofloxacin (30%), Cefipime (23%), Ceftazidimern(33%), penicillin-g (100%). The highest resistance seen in penicillin-g (100%)rnand the lowest resistance in Imepenem (0%).