International Journal of Medical and Health Research

International Journal of Medical and Health Research


International Journal of Medical and Health Research
International Journal of Medical and Health Research
Vol. 2, Issue 9 (2016)

D-Limonene ameliorates diabetic neuropathic pain in rats


Swati Sharma, Nitin Bansal

The present study aimed to explore the role of d-limonene in the management of diabetic neuropathy. Adult Wistar rats (either sex, 200-250 g) were divided into 6 groups (n=6). Diabetes was induced in overnight fasted rats by a single intraperitoneal injection of streptozotocin at a dose of 50 mg/kg body weight freshly dissolved in 0.1 mol/L citrate buffer, pH 4.4. D-limonene was administered in 2 doses (100 and 200 mg/kg; p.o.) to separate groups of rats for 35 successive days daily. Gabapentin (100 mg/kg; i.p) served as standard drug. The neuropathic activity was evaluated in streptozotocin induced diabetic neuropathicrats using Cold andhot water immersion test and tail flick test. After wards, the animals were sacrificed; sciatic nerve is isolated, homogenized and centrifuged for TBARS, GSH, nitrite, catalase and protein estimations. STZ induced diabetic neuropathy caused the decrease in body weight, decrease in tail-flick latency time in radiant heat apparatus, decrease tail-withdrawal latency in tail-immersion (warm water) test, decrease allodynic response in tail-immersion (cold water) test. Furthermore STZ induced diabetic neuropathic rats expressed higher sciatic nerve TBARS and nitrite levels and lower sciatic nerve GSH and catalase levels. D-limonene significantly attenuated (p<0.05) the behavioral and biochemical alterations produced by STZ induced diabetic neuropathy in rats. Catalase, GSH and total protein levels were restored (p< 0.05) in the d-limonene treated animals. D-limonene treated rats showed a significant (p< 0.05) decrease in nitrite and TBARS level. In conclusion, d-limonene may prove to be useful remedy for the management of diabetic neuropathy owing to its possible antioxidant properties.
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How to cite this article:
Swati Sharma, Nitin Bansal. D-Limonene ameliorates diabetic neuropathic pain in rats. International Journal of Medical and Health Research, Volume 2, Issue 9, 2016, Pages 34-39
International Journal of Medical and Health Research International Journal of Medical and Health Research