Study of multidrug resistant nonfermenting gram-negative bacilli
Dr. Ahsan Hamidi, Dr. Shamim Ahmad, Dr. SN Singh
Introduction:Non-fermenting gram-negative bacilli (NFGNB) are ubiquitous in the nature. They are considered as environmental contaminants and most of them have emerged as important no socomial pathogens. So this study was undertaken to identify the various NFGNB with its antimicrobial susceptibility pattern of specimens from patients admitted to Intensive care unit in Government Medical College and Hospital, Bettiah. Methods: Total 107 NFGNB were identified during the study period of March 2014-Nov 2015. All isolates were identified using a standard conventional method like motility, oxidase production; oxidation-fermentation test etc. and failing to acidify butt in triple sugar iron medium. Antibiotic susceptibility testing was performed with the help of the Kirby-Bauer disc diffusion method and (Metallobetalactamase) MBL detection done by DDST and Modified Hodge test. Result: P. aeruginosa (16%) was the commonest isolate obtained followed by A. baumannii (13.5%). NFGNB were maximum susceptible to imipenem (80.4%), piperacillin tazobactum (71.9%) and amikacin (52.3%). P. aeruginosa and A. baumannii were 90% and 60.6% sensitive imipenem and 85% and 54.5% to piperacilli-tazobactum. 19.6% MBL producers were obtained in all nonfermenters. Maximum MBL production was obtained in Acinetobacter spp. (39.4%) followed by S. maltophilia (20%) and P. aeruginosa (10.0%) Interpretation & conclusion: Multidrug resistance infection in ICU is emerging; we also obtained 19.6% MBL producers in all NFGNB. Local antibiotic policy should be generated to prevent the emerging MDR in NFGNB.
Dr. Ahsan Hamidi, Dr. Shamim Ahmad, Dr. SN Singh. Study of multidrug resistant nonfermenting gram-negative bacilli. International Journal of Medical and Health Research, Volume 3, Issue 2, 2017, Pages 166-169