Objective: The present study was designed to investigate the thrombolytic activity of methanolic extract of leaves of Mesua ferrea. Method: Methanolic extract of Mesua ferrea.Was used to evaluate various phytochemical methods. Thrombolytic effect of the fraction was investigated in clot lysis experiment. Result: The extract exerted 36.32% lysis of the blood clot in thrombolytic activity test while 85.45% and 7.5% lysis were obtained for positive control (streptokinase) and negative control respectively. So, the extract possessed considerable thrombolytic activity. Conclusion: Our present investigations indicate that methanolic extract of leaves of Mesua ferrea exhibited considerable thrombolytic activity. There may be many pharmacologically active constituents in the fraction. So, extensive pharmacological and phytochemical experiments are essential.