Comparative genome sequence analysis is a powerful technique for gaining insights into any genome of interest. E.coli E24377A is a human pathogen, which causes diarrhea. After the whole genome of two strains of E.coli, the virulent E24377A and non-pathogenic, commensal HS were sequenced; there is a hope that comparing the genomes will allow an identification of the genes responsible for its virulence and thus the development of treatment and control. Several virulent proteins are also being investigated, as virulence factors as potential vaccine or drug targets. Here we describe the comparison between the genomes of two strains of E.coli with few existing genomics databases and tools available in the public domain websites. By comparing nucleotide and protein sequences of the two strains, we investigate the existing differences and similarities.