Background: Analyzer has the capability to detect hemolysis, icterus, and lipemia in samples and produces semi quantitative unit less index values for hemoglobin, bilirubin, or intralipid (serum indices), but these serum index functions are not intended for diagnostic purposes. With increasing amounts of the specific interfering compounds, increasing index values are generated, which are linearly correlated with the amount of interferent.rnAim of our study is to find the icteric index of serum samples and to find its correlation with total bilirubin levels.
Methodology: The study was conducted in the Clinical Biochemistry Department. A total of 779 patients blood samples were collected in the month of January 2016. The XL-640, Transasia analyzer measures the absorbances for icteric index at 480 nm (primary wavelength) and 505 nm (secondary wavelength). From these absorbance values the instrument calculates the serum index value for interference by bilirubin. Total bilirubin was also estimated for the above mentioned blood samples.
Results: Pearson’s correlation coefficient (r) is calculated to find the correlation between total bilirubin and icteric index which is 0.743.This suggests a strong positive correlation between the two. Higher the serum bilirubin levels, greater was the icteric index.
Conclusion: The icteric index is a semi quantitative test, it can’t be used for decision-making but to detect patients with total bilirubin values above the reference range, it can be safely utilized by laboratories.