Background: Globally, in vitro culture of all life stages of Plasmodium falciparum, existing in the human hosts,is being successfully accomplished under disparate environmental conditions for a variety of studies. Methods: It is universally accepted that culturing of the parasites in a laboratory is challenging being expensive and resource intensive besides the risk of contamination. Different laboratory conditions give varying results in terms of development of mature gametocytes. We have reviewed globalpublished literature on these conditions and analysed them in-depth. Results: Of all the factors analysed, the level of initial parasitemia appears to be most critical in obtaining maximum yield of mature gametocytes. Conclusion: This review explores the key factors involved in lab culture of the gametocyte and its finding can assist in optimising the lab resources for maximal gains. Suggestions have also beenmade on the plausible areas of future research in Plasmodium falciparum gametocytogenesis.