Today 80% of the world's 1 billion smokers Iive in low- and middle-income countries. If trends continue tobacco-related deaths will increase to 8million per year by 2030 with 80% of those deaths in low- and middle income countries. The present study aims to evaluate biochemical oxidative stress markers, serum lipid profile and liver function test in smokers and non-smokers patients. The biochemical oxidative stress markers estimated were malondialdehyde, vitamin C and serum uric acid levels, plasma lipid profile and liver function test Plasma levels of total bilirubin, alkaline phosphatase (ALP), alanine aminotransferase (ALT), aspartate aminotransferase (AST), total protein and albumin were estimated in all subjects The results of present study demonstrate that smoking significantly increases oxidative stress. In our study we found that level of cholesterol, triglyceride, LDL and VLDL is significantly increases in smoker as compared to nonsmoker while HDL decreases significantly in smoker, level of liver enzymes (AST, ALT, ALP) were significantly increased in smokers while level of serum bilirubin, total protein and albumin decreases in smokers as compared to nonsmoker.so we conclude that chronic smoking leads to oxidative stress and These effects could lead to alteration in liver function test. Increased plasma lipid profile shows increased cardiovascular risk.