Hypovitaminosis D is invented to be related with diabetes mellitus and metabolic syndrome. Deficiencies in pancreatic β cell function and/or insulin compassion are frequently present, to develop glucose intolerance or type 2 diabetes mellitus. There are adding evidence that vitamin D may influence these mechanisms. The study has planned in, Shri Ramkrishna Institute of Medical Sciences & Sanaka Hospitals. The 20 Diabetic patients and 20 controlled normal patients were enrolled in to the study. The age group of the patients are from 30-70 years. Thereare very inadequate studies reported of vitamins D3 association with insulin resistance. The aim of present study is to compare levels of the Vitamin D3 in normal and diabetes patients. The data from the normal study group suggests that the mean 25(OH)D levels are found low in the diabetes patients as compared to the normal patients. Also the levels of than the 20 ng/ml showed the deficiency. Hence in the enrolled study group showed the vitamin D3 deficiency. Hence form the current study determines that the mean 25(OH)D levels in type 2 diabetes subjects is lower than normal individuals.