This study has been performed to determine the best parameter in metric diagnosis of sexing by using discriminant function analysis on adult human tibia. Upper end of 99 normal, dry, adult human tibia of known sex (63 males and 36 females) were measured by using osteometric board and manual vernier callipers. Mean, standard deviations and t – value was calculated to determine the percentage accuracy. The percentage accuracy of various tibial parameters are – Length of tibia (68%), Total transverse diameter (82%), Transverse diameter of Medial condyle (73%), Transverse diameter of Lateral condyle (70 %), Antero- posterior diameter of Medial condyle (75%), Antero posterior diameter of Lateral condyle (79%) and Antero-posterior diameter of tibia (73%). From the above study, it can be concluded that according to the percentage of accuracy - Total transverse diameter (TTD) and Antero posterior diameter of Lateral condyle (APLC) are considered as best parameters of sex determination.