The study was under taken to assess the knowledge and knowledge on practice among prenatal mother, regarding prevention of HIV and AIDS in selected urban, Bangalore. 50 prenatal mothers were selected through purposive sampling technique and the tool was administered. Through interview schedule, collected data was analyzed that 48% of prenatal mothers on moderate knowledge and 71% of them moderate knowledge on practice regarding HIV prevention. The was concluded that significant association was found on knowledge with selected demographic variable in education and knowledge on practice such as age, education and occupation hence study was found significant as stated assumption.