Introduction: Liver function tests do vary with various factors like race/ethnicity, environmental, life styles, metabolic, physiological changes with advancing age and gender. Clinicians need to know these variations in reference interval when considering the presence of liver disease. There is scarce data of reference interval of liver function tests in north Indian population which have drastically different food habits, life style, exposure to environmental conditions as compared to the population of Southern and Eastern India. Material and Methods: Apparently healthy medical and paramedical professionals and healthy volunteer donors coming to the blood bank were included in the study and studied for eight liver function parameters. Results: 1302 subjects were screened for the study.265 subjects were excluded as 73 were diabetics (fasting