Pleural fluid of infectious etiology is major cause of morbidity & mortality among patients. Aim of the study is to study bacteriological profile in Pleural fluid and to know its antibiotic susceptibility pattern. Material & Method: Pleural fluid sample were collected by standard procedure using aseptic precautions. All specimens of Pleural fluid from Nov 2017 to Oct 2018 that came for culture & sensitivity to Microbiology department in tertiary care teaching hospital Solapur, were included in the study. All the fluids were subjected to gram staining for provisional report and then inoculated on culture media and incubated overnight at 37ºC. Growth if any was noted and isolate was identified using standard protocols. Antibiotic susceptibility testing was done using Kirby bauer disk diffusion method. Results: Total sample of Pleural fluid received from Nov 2017 to Dec 2018 were 120 out of which 42 (35%) showed growth while 78 (65%) samples were sterile. Culture positivity rate was 35%. Majority of isolates were Gram negative bacilli 34 (80.95%) of which most common was Acinetobacter baumanii 14 (33.33%) followed by E. coli 6 (14.28%), Klebsiella pnemoniae 4 (9.52%), Enterobacter aerogens 4 (9.52%), Citrobacter koseri 4 (9.52%) and Pseudomonas aeruginosa 2 (4.76%). Among gram positive spectrum, Staphylococcus aureus were isolated from 8 (19.04%) samples. Of which MRSA were 6 (14.28%) and MSSA were 2 (4.76%). Males 25(59.52%) were more commonly affected then females 17 (40.50%). Most common age group affected was 31-45 years in males while in females most common age group affected was 16-30 years. Overall among gram negative bacteria maximum resistance was noted for Ampicillin 68.75%, Ciprofloxacin 68.75%, Cefotaxim 68.75% followed by Imipenem 53.13%.High sensitivity was seen for Amikacin 64.70% followed by Piperacillin-tazobactam 58.82%. Overall among gram positive organisms 100% sensitivity was seen for Vancomycin, Gentamicin & Ciprofloxacin. Sensitivity for Erythromycin, Clindamycin & Cotrimoxazole was 75%respectively. Resistance to Cefoxitin was noted in 75% of gram positive isolates.