Objective: detection of hepatitis C virus antibodies in serum samples can be done by different methods. However, choosing between them is still a confusing matter. So, this study aimed to make a comparison between some serological methods for detection of hepatitis "C" virus antibodies in Taif Governorate to identify the most sensitive, specific and accurate technique to facilitate the correct diagnostic process for HCV. Methods: a total number of 200 blood samples were collected in this study during the period from June 2017 to December 2017 in Taif Governorate for detection of anti-HCV by ELISA, CLIA and RIBA techniques. Results: the results declared that CLIA was better than ELISA for the detection of anti-HCV with high sensitivity (98.59%), specificity (89.65%), accuracy (96%) and precision (96%). The RIBA has been used in this study as a confirmatory technique for detection of antibodies to HCV in blood samples. Conclusion: the results of this study indicate that CLIA was more sensitive, specific and accurate than ELISA for identifying anti- HCV and confirmed by RIBA.