Enterococci are the member of healthy human intestinal flora but are also leading causes of highly antibiotic resistant infections. Serious enterococcal infections are often difficult to treat since the organism has a tremendous capacity to acquire resistance to penicillin, high concentration of aminoglycoside and vancomycin. Aim of the study to check the prevalence rate of enterococcus species in our hospital. A total 125 enterococcus were studied, species identification was done by conventional methods. Antibiotic susceptibility testing was by done by Kirby-Bauer disc diffusion method. Vancomycin resistance were detected by disc diffusion and E-test MIC method. In 125 clinical isolates of Enterococcus, 88 species are Enterococcus faecalis and 37 species are Enterococcus faecium. Out of 125 enterococcus isolates 16 were Vancomycin resistant. Regular screening of enterococcal isolates for vancomycin resistance detection should be implemented. It is very important to implement infection control measures, screening of health care workers in intensive care units which can control spread of multidrug resistant enterococci.