Neonatal sepsis is a type of neonatal infection and specifically refers to the presence in a new born baby of a bacterial blood stream infection (BSI) (such as meningitis, pneumonia, pyelonephritis, or gastroenteritis) in the setting of fever. The literature revealed that early identification is a significant to diminish morbidity and mortality of neonatal septicemia. Based on this findings the present study is planned to evaluate the etiological profile of neonatal septicemia cases and their antibiotic sensitivity pattern for planning strategy for the management of these cases. From the above data it is concluded that E. coli is the most common cause of neonatal sepsis in patients admitted. Most of the empirical regimens/ commonly using antibiotics like Ampicillin, Amoxicillin, Cefotaxime and Gentamycin are highly resistant against both Gram positive and Gram-negative pathogens causing neonatal sepsis. Neonatal septicemia is a life threatening emergency. The study of etiological profile and their antibiotic sensitivity pattern plays a significant role.