The indiscriminate, haphazard and half-hearted use of antibiotics and poor follow up of the patients have resulted in persistent changes in the bacteriological pattern of the disease, the advent of new antimicrobials, anti-inflammatory and anti-histamine agents make an evaluation of bacterial flora of CSOM important. Increasing resistance of microorganism to the drugs has been reported. This leads to development of serious complications. Hence the present study was planned to find out the predominant organisms in the locality and the antibiotic susceptibility of the bacteria. The present study was planned in the Department of Microbiology, mata Gujri memorial medical college and Lions seva kendra Hospital from Jan 2000 to August 2000. Total 50 cases of Chronic suppurative otitis media of all age groups and both sexes attending outpatient department and those admitted in ENT wards were selected randomly for the study. The bacteriological agent associated with CSOM has not changed significantly in our environment. However, there is a gradual decline in their sensitivity pattern to a number of antibiotics. The important factor responsible for development of resistance is inappropriate duration of treatment and dose of antibiotics. Judicial use of antibiotics is necessary for the prevention of development of antibiotic resistance.