Introduction: Bilirubin is a tetrapyrrole pigment formed out of the combination of heme of the hemoglobin of blood cells. Billi Test is a simple method for cutaneous estimating total serum bilirubin levels in noninvasive, quick and painless to is used. Method: The present descriptive analytical study was carried out on neonates with hyperbilirubinemia who were hospitalized in Neonatal and NICU Wards at Amir Almomenin (AS) Hospital in the first half of 2013. Findings: Neonates diagnosed with hyperbilirubinemia admitted in pediatrics and NICU of Amiralmomenin Hospital of Zabol were studied in the present research; 64 neonates (53.3%) were boys and 56 neonates (46.7%) were girls. The average weight of newborns was 2.4±0.7 kg and the mean gestational age was 35.4±2.8 weeks. (P<0.05) The mean rate of cutaneous bilirubin turned out to be 14.4±2.8 mg/dl among infants diagnosed with hyperbilirubinemia examined in the present research. Conclusion: According to the results of our study and other studies, it seems that the Bilicheck device has the necessary accuracy as a measuring and monitoring device for neonatal jaundice and can be an appropriate alternative for serum bilirubin measurement in neonates with jaundice.