This study was done in the department of Anatomy, Patna Medical College & Hospital, Patna. The main aim is to study the Brunner’s gland is to make a comparative study of different types of mucin secreted by Brunner’s gland of few mammals by applying various histochemical techniques. These histological and histochemical observations on Brunner’s gland was done in guinea pig, albino rat, rabbit and goat, which belongs to different order and have different food habits. Hematoxylin and eosin staining the secretory cells of Brunner’s gland in case of guinea pig, albino rat and goat to be typically mucous in nature whereas in rabbit the glands were of mucous serous type in guinea pig Brunner’s gland secreted mixture of acid and neutral mucin. In albino rat mucous acini secrete moderate amount of acid mucin. In goat Brunner’s gland secreted negligible amount of acid mucin. Common finding in the secretion of Brunner’s gland of the above mammals was the absence of glycogen. Dietary habits and order of these animals have no impact on the type of secretion. The present study provides a base line for further histological studies on the mucin.