Background: Candiduria is one of the most common symptoms of urinary tract infections caused by several species of Candida. Several antifungal drugs are available to treat such candida infections. During the last decade, resistance to antifungals especially in non-albicans species has increased. Objectives: The present study aimed to evaluate the speciation and antifungal susceptibility profile of Candida isolates from urine samples. Materials and Methods: A total of 100 Candida spp. isolated from urine samples were included in the study. Speciation of Candida was done by conventional methods and colony color on CHROM agar. Antifungal susceptibility testing of the isolates was performed by disc diffusion method on Glucose Methylene Mueller - Hinton agar (GM-MH) Results: Candiduria was more common in females. In both the sexes maximum patients belong to age group 51-60 years. Urinary catheterization followed by the use of broad spectrum antibiotics and diabetes mellitus were the major risks for the development of candiduria. Out of 100 isolates, 36(36%) were C. albicans whereas 64 (64%) belonged to Non albicans Candida (NAC) spp. Among NAC spp. C. tropicalis was the major isolate. Candida species showed maximum sensitivity to Amphotericin B 95%, followed by Ketoconazole 69%, Conclusions: Candiduria is becoming an important nosocomial infection. Incidence of non Candida albicans was more than Candida albicans. NAC species are more resistance to antifungal as compared to C albicans