Background: Urinary tract infection (UTI) is a significant and a serious health affecting problem encountered both in the community and hospital settings each year worldwide. The causative pathogen and their susceptibility pattern varies from region to region and changes with time. Objective: To investigate the etiologic agents and its antimicrobial susceptibility pattern of UTI in a tertiary care hospital Material and Methods: Clean catch, mid-stream urine samples were received in sterile universal containers. Urine culture was done by a semi-quantitative method. Antibiotic sensitivity was done on bacterial isolates according to the Clinical and Laboratory Standards Institute CLSI guidelines for disc diffusion susceptibility test. Results: Significant Bacteriuria was present in 54.66% sample and 45.34% were sterile. The most common pathogens isolated among gram negative bacteria were E.coli (57%) while in gram positive cocci Enterococcus species was the most prevalent 26(8.09%) Gram negative organisms showed highest sensitivity to nitrofurantoin (65.66%), followed by amikacin (56.22%). Gram positive organisms showed highest sensitivity to linezolid (83.33%) followed by vancomycin (75.00%). Conclusion: Constant evaluation of the antibiotic sensitivity pattern of UTI pathogens for commonly used antimicrobial agents in a particular environment should be carried out regularly.