Aim: To study the incidence & type of microdeletions in patient with infertility by Y Microdeletion by Polymerase chain reaction (PCR) studies. Materials and Methods: In 763 patients aged from 21-47 years with suspicion of Y microdeletion PCR based Y microdeletion studies carried. 6-8 ml of peripheral blood was collected in EDTA Vacutainers and the genomic DNA were extracted and analysed by PCR. Results: Microdeletion was observed in 30 patients with a total deletion rate of 3.9%. Of these deletions, AZFc deletions was seen in 2.6%, AZFb deletions in 0.8%, microdeletion of AZFb and AZFc in 3 patients with a deletion rate of 0.4%, 1 patient showed deletion in AZFa, AZFb and AZFc with a deletion rate of 0.1%. Conclusion: Knowing the genetic status of Y chromosome in male infertile individuals currently in the era of advanced assisted reproductive technology (ART) can be very helpful to the couple.