Septicemia is one of the important causes of mortality and morbidity in neonates and children. Aim of this study was to assess the frequency and antibiogram of microorganisms isolated from blood cultures. The study was conducted on blood cultures obtained from NICU & PICU during the period of January 2016 to October 2017. Isolation, species identification and antibiotic susceptibilities were done by automated Bact Alert and Vitek 2C methods. A total of 182 samples were received during the study period out of which 19 (10.44%) were culture positive. Among 19 culture positive samples, children were 10 and neonates were 09. The commonly isolated organism was Coagulase Negative Staphylococci (CONS) (57%), followed by Candida albicans (21%), Acinetobacter spp. (10.5%), Escherchia coli (5.2%) and Klebsiellae pneumonia (5.2%). The present study contributes information on the prevalence of microorganisms in blood stream infections and also indicates the presence of fungemia, which highlights the need for periodic surveillance of etiologic agents.