Background of the study: Hospitals produce a verity of medical waste which is responsible for serious health hazards. Now today nursing is considered as a professional discipline that includes the art of applying scientific knowledge to practice. So it is very important to make awareness among future nurses and midwifes regarding biomedical waste management. Objectives: To assess the effectiveness of planned teaching program on knowledge regarding practice of biomedical waste management among final year regular undergraduate Nursing and Midwifery students of Wollega University, Ethiopia Methodology: The design adopted is one group pre-test and post-test pre-experimental design, the samples were chosen by purposive sampling techniques, consist 100 from final year regular undergraduate Nursing and Midwifery students of Wollega University, and a structured knowledge questionnaire is given to assess the knowledge on practice of biomedical waste management. Which followed by a planned teaching program on biomedical waste management and administering the same questionnaire to assess the post test. Results: The overall mean post-test knowledge score regarding practice of biomedical waste management (14.51±4.9) was significantly higher than overall mean pre-test knowledge score regarding practice of biomedical waste management score (11.67±2.88) and the obtained ‘t’ value was 11.12, which was higher than the table value. There was no significant association found between the pre-test knowledge score with the demographic variables of students.