Siddha system of medicine finds important place in the management chronic ailments including cancer. In vitro studies using a variety of cancer cell types have shed light on the mechanistic insights into the anticancer effect of various regimens including Siddha medicines. Pancha Paasana Chendhuram (PPC) is a herbo-mineral formula claimed to possess significant anticancer activity in Siddha system. In the present study, PPC was investigated for its apoptosis mechanism using human cervical cancer cells. PPC up-regulated Bax and p53 and down-regulated BCl2 mRNAs expression in human cervical cancer cells (HeLa cells).