Aim: This study was undertaken to logically and intricately evaluate and compare antimicrobial effects of BioPure MTAD (MTAD) and Niclor 5 irrigating solutions as observed against planktonic and biofilm forms of E. faecalis and Candida albicans. Materials & Methods: Total 45 recently extracted non carious permanent maxillary incisors teeth of both genders were studied. Biomechanical preparation of the intended canals was attempted precisely as mentioned in the standard literature and books by renowned researchers. Authors had intended to limit their biomechanical preparation up to 40 K sized file only. Group I (BioPure MTAD [MTAD]), group II (Niclor 5). The third group was kept as control group (sterile distilled water). The antimicrobial effect in each group was observed, documented, statistically analyzed and compared. Statistical Analysis and Results: Statistical analysis was completed using statistical software Statistical Package for the Social Sciences version 21. The measured mean for group I, II and III was 25.57, 23.42, 26. 58 respectively. Standard deviation was noted least for group II (0.142) and maximum for group I (0.185). Standard error was noted minimum for group I (0.123) while it was maximum for group III (0.571). Candida albicans colony count was highest in group III and lowest in group II. Particularly it was 4.98±0.34 in group I, 2.12±0.67 in group II, 11.09± 2.43 in group III. E. Conclusion: Niclor 5 exhibited to be remarkably efficient against colonies of planktonic and biofilm forms of E. faecalis and Candida albicans. Therefore Niclor 5 may be clinically employed as an effective root canal irrigant. In this study, the effectiveness of two different irrigating solutions was tested, wherein Niclor 5found to be more efficient in reducing bacterial count.