During short term painful procedures in neonates (heel lancing), non-pharmacological pain relieving interventions are effective, economical and safe as compared to pharmacological pain relieving interventions. Non- pharmacological procedures such as oral dextrose solution, nonnutritive sucking (NNS), swaddling, breast feeding, facilitated tucking and skinto-skin contact had been effectively used for amelioration of minor painful procedures in neonates. Hence based on above findings the present study was planned for Evaluation Comparative Study of Analgesic Effect of Breast Feeding and Dextrose of the Heel Lance in Neonates. The present study was planned in Department of Pediatrics, Anugrah Narayan Magadh Medical College and Hospital, Gaya, Bihar, India. The study was conducted from the June 2018 to December 2018. In the present study 25 Neonates 0 to 28 days of age (with gestational age 34 weeks to 42 weeks) who are undergoing heel lance procedure for blood sugar estimation and those babies on exclusive or partial breast feed were enrolled. The data generated from the present study concluded that none of the children in breast feeding group showed features of severe pain. Lesser mean score in breast feeding group signifies the importance of breast feeding during painful procedures as compared to dextrose. It is clear in our study that breast feeding more effectively reduces the pain among neonates in comparison to dextrose during heel lance.