Evaluation of latex agglutination test (LAT) and efficacy in diagnosis of pyogenic meningitis in children
Dr. Kisoar Farhana Keya, ASM Nawshad Uddin Ahmed, Dr. Amal Kanti banik
Pyogenic meningitis is one of the most common and devastating disease of Central Nervous System, especially in children. We carried out an observational study with the aim was Evaluation of Latex Agglutination Test Efficacy in Diagnosis of Pyogenic Meningitis in children under 5 years during December 2012 to May 2013 in Paediatrics department of Dhaka Shishu (Children) Hospital, Dhaka, Bangladesh. We took our participants who were clinically suspected cases of pyogenic meningitis in children below 5 years of age admitted in our selected hospital. All the data were collected and recorded systematically in a questionnaire and entered into computer software SPSS (Statistical package for social sciences) version 20.0 edited and analyzed. Sensitivity and specificity of Gram stain, CSF latex were calculated. CSF cell count, Gram stain, Culture, LAT and Blood culture were the main outcome variables. A total of 100 cases of suspected meningitis in the age group below 5 years selected the study population. Majority of the patients belonged to ≤12 months. The most common clinical presentation was fever (100.0%) with median duration of 2 days. Ninety six (96) patients had convulsion and the median duration of continuation of convulsion was 1 day. Thirty eight patients had vomiting and median duration of continuation of vomiting was 1 day. A total of 100 clinically suspected cases of acute bacterial meningitis, laboratory confirmed cases were 36(36.0%), out of which 14(38.9%) cases were CSF culture positive, 10(27.8%) were Gram stain positive and 36(36.0%) were Latex agglutination test positive. Most frequently isolated organism were Streptococcus Pneumoniae 34(94.4%). The validity of test of serology (LAT) sensitivity was 100.0% and specificity was 74.4%, Gram stain sensitivity was 71.4% and specificity 100.0%. CSF culture is the ‘Gold standard’ and positive in 38.9% cases, but Latex agglutination test was 100.0% sensitive for detection of Antigen in CSF. In the conclusion, we can say. LAT can be a valuable tool in early diagnosis of acute pyogenic meningitis cases even after administration of antibiotics.
Dr. Kisoar Farhana Keya, ASM Nawshad Uddin Ahmed, Dr. Amal Kanti banik. Evaluation of latex agglutination test (LAT) and efficacy in diagnosis of pyogenic meningitis in children. International Journal of Medical and Health Research, Volume 5, Issue 3, 2019, Pages 18-21