Aims and Objectives: The purpose of this study was to design a standard questionnaire for assessing job satisfaction of dentists. Materials and Methods: In order to prepare the questions bank, we reviewed the available resources related to the subject and determined the validity of the appearance and validity of the content, the questionnaire was reviewed by the experts. To examine reliability, Cronbach's alpha coefficient calculated, and for validity, Content Validity Index (CVI) Content Validity Ratio (CVR), and Inter-Class Correlation (ICC) coefficient was reported. Result: The questionnaire had 12 domains: Income, Sense of sympathy and altruism, social respectful, work Independence, Communication with colleagues, staff and patients, work skills, workload, self-satisfaction, Feel the ability, Environment of Workplace, Stress. The questionnaire was designed in 47 questions. The Cronbach's alpha coefficient was 0.813. CVI and CVR for all questions were estimated between 25 -75% and 80-95% respectively. The ICC was calculated at two times equal to 0.97 (p <0.001). Conclusion: Considering the use of standard techniques for designing the research tools and the results on validity and reliability indices, it seems that this questionnaire could be used as a reliable tool for determining the job satisfaction of dentists.