Background: Human immunodeficiency virus (HIV) has infected several million individuals in India. Various interventions have been implemented for early detection and prevention of transmission of HIV infection. Aims and Objectives: To study the clinical profile of HIV infection and compare it with the duration of disease. Materials and Methods: Hundred HIV patients were studied at JA Groups of Hospitals, G.R.M.C Gwalior, Department of Medicine from November 2016 to August 2018.Patients were divided based on the duration of the disease as Group A (≤2 years), Group B (2-5 years) and Group C (>5 years). Different groups were compared with the clinical profile of HIV patients. Detailed sociodemographic profile along with CD4 count was done. Patients were also classified according to WHO classification stages. Results: Majority of the patients were in the age group of 31-40 years (39%). Male preponderance was recorded (66%). Most common occupation was housewife (33%). Majority of the patients were at high risk sexual behavior (48%) and had duration of therapy of 2-5 years (55%). Among the Group B patients majority were male had primary education, were drivers, belong to urban area, majority had CD4 count of 200-400, and had WHO grading of 1. Conclusion: Early detection is the key to prevent the further spread of the disease. Duration of disease between 2-5 years had significant impact on male patients who had primary education only and had lower quality of life as majorities were drivers. The signs and symptoms strongly associated with HIV infection can be used for vigilance and early detection of HIV.