Bacterial conjunctivitis can be contracted directly from infected individuals or can result from abnormal proliferation of the native conjunctival flora. Most causes of conjunctivitis are benign, with a self-limited process; however, depending on the immune status of the patient and the aetiology, conjunctivitis can progress to increasingly severe and sight-threatening infections. Chronic conjunctivitis is usually a source of frustration to both the patient and the ophthalmologist. The role of the emergency physician is to separate the conditions requiring more vigorous treatment and referral from those that can be handled satisfactorily in the emergency department. Hence based on above findings the present study was planned for Assessment of Different Organism Responsible for Acute Bacterial Conjunctivitis Cases. The present study was planned in Department of Ophthalmology, Patna Medical College & Hospital, Patna, Bihar, India. The study was conducted from December 2018 to February 2019. In the present study 25 cases of the acute conjunctivitis referred to Department of Ophthalmology were enrolled. A complete external examination of each eye including lids, conjunctiva, cornea, preauricular and submandibular lymph nodes, slit lamp bio-microscopy of the anterior segment, application of Fluorescein to the ocular surface and Schirmer's test where needed. Condition of the lids is noted for any evidence of oedema, blepharitis, mucous crusts, madarosis, tylosis, trichiasis, ectropion, etc. Conjunctiva is examined for evidence of congestion, chemosis, ulceration, petechial haemorrhages, membrane formation, papillae, follicles, nodules, scarring, discoloration etc. The data generated from the present study concludes that acute conjunctivitis is the common problem seen worldwide. It is generally seen in the patients with low socioeconomic status. Prevention of any disease is better than cure which happens to be very true especially regarding to acute conjunctivitis. Staphylococcus aureus, Streptococcus pneumonae, Hemophilus influenzae and Staphylococcus epididymis were most common bacteria seen in our study.