Expression of enzyme acid phosphatase and effect of potassium iodide on its production in mycelial form of Sporothrix schenckii
Context: Sporotrichosis is a varied disease caused by a single dimorphic fungal species, Sporothrix schenckii (S. schenckii).Aims: This study describes the in-vitro effect of potassium iodide (KI) on the enzyme acid phosphatase produced by the filamentous form of S. schenckii. Methods and Material: A master culture of S. schenckii was prepared in YNB (Yeast nitrogen base) medium and was incubated at 25ºC (mould). KI was added into the YNB medium in increasing concentrations. One mL suspension of master culture was inoculated into each bottle and incubated at 25ºC for different time period, 4th day (early-log period), 9th day (mid-log period), 14th day (peak of growth) respectively. After centrifuging, a 5% homogenate was prepared that was used for acid phosphatase enzyme assay.Results: The mean acid phosphatase level of control specimen was 25.0 ± 4.53, 39.9 ± 4.07 and 84.1 ± 5.15 μg on day 4, 9 and 14 respectively. The mean acid phosphatase level of test specimens was ranged from 14.0 ± 2.44 (KI 6.4) to 25.0 ± 3.08 (KI 0.05), 10.5 ± 1.61 (KI 6.4) to 44.0 ± 4.44 (KI 0.05) and 8.1 ± 1.62 (KI 6.4) to 80.0 ± 4.68 μg (KI 0.05) on day 4, 9 and 14 respectively. Conclusions: At the entire test concentrations mean value was lower as compared to control. The low activity of the enzyme acid phosphatase indicates that KI has inhibitory effect on the growth of S. schenckii (mould) and has led to decrease in the activity of the enzyme.
Rajesh Bareja. Expression of enzyme acid phosphatase and effect of potassium iodide on its production in mycelial form of Sporothrix schenckii. International Journal of Medical and Health Research, Volume 5, Issue 8, 2019, Pages 203-206