S. aureus produces various infections ranging from minor skin infection to life-threatening infections. Methicillin resistant staphylococcus aureus (MRSA) strains has emerged as one of the commonest nosocomial pathogens. Infection with MRSA strains is likely to be more severe, requires longer hospitalisation and is associated with considerable increase in morbidity, mortality and health care costs. MRSA infection is a serious concern, because of its resistance to commonly used antibiotics. Many of the MRSA isolates are becoming multidrug resistant and are showing susceptibility only to glycopeptides such as vancomycin. At present, they are showing low level resistance even to vancomycin. Hence present study was planned to evaluate the Antibiotic susceptibility pattern among clinical isolates of staphylococcus aureus with special reference to vancomycin. The present study was planned in the Department of Microbiology, Nalanda Medical College and Hospital, Patna, Bihar from July 2018 to Jan 2019. Total of 100 non-repetitive clinical isolates of Staphylococcus aureus were isolated from various clinical specimens received in microbiology. These isolates were identified by conventional phenotypic methods such as colony morphology, Gram’s stain, catalase test, slide and tube coagulase test, growth on mannitol salt agar, DNase production, gelatinase production and phosphatase production. The results of the present study highlights the alarming development of resistance to almost all the drugs with a very few exception of drugs used in this study. Non-empirical /inappropriate antibiotic use contributes to the emergence of antimicrobial resistance in bacteria both Gram negative and Gram positive organisms. Vancomycin still remains the mainstay of treatment for serious enterococcal infections, if the strain is found susceptible. The data on local patterns of susceptibility of vancomycin resistant enterococci to newer antimicrobial agents can help in guiding the treatment of these pathogens.