Background: The present study was done to isolate and identify of causative agents of urinary tract infection and their antimicrobial susceptibility pattern. Introduction: Urinary tract infection (UTI) is the second most common infection in human after respiratory tract infection. UTI is more common in females than males. E. coli is most common organism responsible for urinary tract infection. Materials and Methods: A total 1102 urine samples were collected, out of these samples, 463 urine samples were collected from female patients of reproductive age group (15-45 years). Urine samples were inoculated on MacConkey, CLED (Cysteine Lactose, electrolyte deficient) and Blood agar incubated at 37â—¦C for 24 hrs, and colonies were processed according to standard procedures Results: A total 463 urine samples were processed during the study period, from female patients of reproductive age group. 172 (37.1%) had significant bacterial growth, of which 54.65% belonged to 15-25 years of age group followed by 23.25% belonged to 26-35 years and 22.10% belonged to 36-45 years of age group. E. coli are the most common pathogens causing UTI. 44.2% bacterial isolates were identified as E. coli followed by Staphylococcus aureus 18.6%, Klebsiella pneumoniae 11.6%, Coagulase Negative Staphylococci 9.9%, Streptococcus species 5.2%. Conclusion: Prevalence of UTI was seen higher in Group A (15-25) as compared to Group B (26-35) and Group C (36-45). Both Gram Positive and Gram Negative bacteria are responsible for UTI with dominance of Gram Negative bacteria. In case of Gram Negative bacteria, Cephalosporins showed high resistance while the sensitivity rate of Carbapenems was good. Similarly resistance rate of Penicillin, Ampicillin was high in case of Gram Positive organisms and Vancomycin, Linezolid, Nitrofurantoin showed better sensitivity pattern.