Aim: To evaluate the influence of endodontic irrigants on adhesion to pulp chamber dentin. Material and Method: Seventy crowns of bovine incisors were cut to expose the pulp chamber. Specimens were divided into seven groups, according to the irrigant solution used: G1, 0.9% sodium chlorite (control); G2, 5.25% NaOCl; G3, 5.25% NaOCl 17% EDTA; G4, 2% chlorhexidine solution; G5, 2% CHX solution 17% EDTA; G6, 2% chlorhexidine in a gel base; and G7, 2% CHX gel 17% EDTA. After irrigation, Clearfil SE Bond was applied to pulp chamber dentin, followed by Filtek Z250 composite. Six rectangular sticks were obtained from each specimen and dentin/resin interface was tested in tension. Bond strength means were analyzed by ANOVA and Tukey test. Results and Conclusion: There was a significant decrease in bond strength associated to NaOCl, whereas chlorhexidine irrigation showed no effects on adhesion. It was concluded that endodontic irrigants affected differently bond strength to pulp chamber dentin.